SubtiBank SubtiBank
Version comparison:

2018-06-28 10:32:172025-05-15 05:03:14

title

yrvN

rarA

description

similar to the E. coli recombination factor RarA, that is involved in processing recombination intermediates at replication forks

DNA-dependent ATPase, recombination factor, involved in processing recombination intermediates at replication forks

locus

BSU27530

BSU_27530

geneLength

1263

1266

function

DNA recombination

control of the access of the replication machinery to a collapsed replication fork

product

unknown

[[protein|RecA]] accessory protein, DNA-dependent ATPase

names

yrvN

rarA

rarA

yrvN

outlinks

bsu

BSU27530

BSU_27530

Gene

Coordinates

2,812,336 → 2,813,601

2,812,336 2,813,601

Biological materials

Mutant

MGNA-A827 (yrvN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/827 NBRP B. subtilis, Japan]

BKE27530 (Δ[[gene|rarA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT

BKK27530 (Δ[[gene|rarA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT

MGNA-A827 (yrvN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/827 NBRP B. subtilis, Japan]

BKE27530 ([[gene|rarA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT

BKK27530 ([[gene|rarA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT

References

21170359, 21297161

21170359, 21297161, 29947798, 30971308, 30954900, 32117122

Gene

Phenotypes of a mutant

sensitive to H2O2 [pubmed|30954900]

reduced viability of [[gene|rarA]] [[gene|recA]] or [[gene|rarA]] [[gene|recO]] double mutants [pubmed|32117122]

The protein

Catalyzed reaction/ biological activity

inhibits [[protein|PriA]]-dependent initiation of [[category|DNA replication]] [pubmed|29947798]

contributes to error-prone DNA repair, links re-initiation of DNA replication with repair-by-recombination by controlling the access of the replication machinery to a collapsed replication fork [pubmed|30954900]

contributes to the assembly of RecA nucleoprotein filaments onto single-stranded DNA [pubmed|32117122]

The protein

Protein family

[SW|AAA ATPase family] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Effectors of protein activity

ATPase is stimulated by ssDNAdsDNA junctions or dsDNA ends [pubmed|29947798]

ssDNA-dependent ATPase is stimulated by [[protein|SsbA]] bound to ssDNA [pubmed|29947798]